Immunology-Endocrinology-Molecular Biology

The research laboratory of the Institute of Medical Psychology and Behavioral Immunobiology covers a wide spectrum of state-of-the art methods in immunology, molecular biology, and endocrinology.

Head

Univ.-Prof. Dr. rer. nat. Harald Engler
Tel. +49 (0)201 723-83044

Technical staff

Alexandra Kornowski, MTA
Phone: +49 (0)201 723-85694 oder -4505

Dipl.-Biol. Magdalene Vogelsang
Phone: +49 (0)201 723-85694 or -4505

Jana Schäfer, B.Sc Mol. Biol.
Tel. +49 (0)201 723-82861 or -4505

Immunology

Methods:
Flow cytometry, hematology, magnetic cell separation, density gradient centrifugation, cytokine analyses by ELISA and multi-array technology, functional assays (proliferation, cytotoxicity, phagocytosis, apoptosis), cell culture (whole blood, primary cells, cell lines)

Equipment:
Flow cytometer (BD FACSCelesta™ with High Throughput Sampler), multi-array ECL analyzer (MESO QuickPlex SQ 120 MM), hematology systems (Sysmex XP-300, scil Vet abc), point-of-care analyzer (Sysmex/Hitado smart 700/546), blood sedimentation device (Sediplus® S200), clean benches (2x HeraSafe HS18), magnetic cell separators (MidiMACS™, QuadroMACS™), CO2 incubators (HeraCell 150i, HeraCell 240), microplate reader (BMG Labtech FLUOstarOPTIMA™), microplate washer (Thermo Scientific Wellwash™), centrifuges (Hettich Rotanta 460R, Micro 200R), ULT freezer (Thermo Forma 900)

Molecular biology

Methods:
Purification/isolation and quantification of nucleic acids and proteins, RT-PCR, real-time quantitative PCR, gene expression analysis and genotyping, gel electrophoresis, Western blotting

Equipment:
Real-time quantitative PCR system (Applied Biosystems 7500 Fast), thermocycler (Applied Biosystems 2720), UV-Vis spectrophotometer (NanoDrop™ One), photometer (Eppendorf BioPhotometer™), Western blotting system (Bio-Rad Trans-Blot Turbo™), gel electrophoresis system (Bio-Rad), µMACS™ separator for proteins and RNA, centrifuges (Hettich Universal 320R, Eppendorf 5415R), ULT freezer (Eppendorf CryoCube F101h)

Endocrinology

Methods:
Quantification of steroid und peptide hormones in plasma/serum, CSF, and saliva by enzyme-linked immunosorbent Assay (ELISA), luminescence immunoassay (LIA), and multi-array technology, quantification of salivary alpha-amylase (sAA) activity by enzymatic assay, development of a smartphone application for the monitoring of the cortisol awakening response (SalivApp).

Equipment:
Multi-array ECL analyzer (MSD MESO QuickPlex SQ 120 MM), microplate reader (BMG Labtech FLUOstarOPTIMA™), microplate washer (Thermo Scientific Wellwash™), centrifuges (Hettich Rotanta 460R, Micro 200R), ULT freezer (Thermo Forma 88000, Haier Biomedical ULT).

About us

The Institute of Medical Psychology was founded in 1978 and is one of the pre-clinical Departments of the Essen University Hospital.

Address

  • Institut für Medizinische Psychologie und Verhaltensimmunbiologie Universitätsklinikum Essen
    Hufelandstr. 55
    45122 Essen
    Germany

Contact

  • +49 201 723 4501

  • +49 201 723 5948

  • Send E-Mail